Description：Rabbit polyclonal antibody to AKTImmunogen：KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human AKT. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IH (1/100 - 1/200)Gene Symbol：AKT1Alternative Names：PKB; RAC; RAC-alpha serine/threonine-protein kinase; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; Proto-oncogene c-Akt; RAC-PK-alpha Entrez Gene (Human)： 207; Entrez Gene (Mouse)： 11651; Entrez Gene (Rat)： 24185; SwissProt (Human)： P31749; SwissProt (Mouse)： P31750; SwissProt (Rat)： P47196; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of AKT expression in HeLa (A), A549 (B), Raw264.7 (C), NIH3T3 (D), mouse lung (E), PC12 (F), rat heart (G) whole cell lysates. (Predicted band size: 55 kD; Observed band size: 60 kD)
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  Immunohistochemical analysis of AKT staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.