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CPA1037
  • Western blot analysis of AKT expression in A549 (A), mouse brain (B), rat lung (C) whole cell lysates. (Predicted band size: 55 kD; Observed band size: 60 kD)
  • Immunohistochemical analysis of AKT staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Product Name:Anti-AKT Antibody
Cat No:CPA1037
Source:Rabbit
Reactivity:H, M, R, B, P, S
Applications:WB, IH, IP
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Size
Price(USD)
200 μl
350
100 μl
220
30 μl
110
50 μl
150
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Description:Rabbit polyclonal antibody to AKT
Immunogen:KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human AKT. The exact sequence is proprietary.
Purification:The antibody was purified by immunogen affinity chromatography.
Clonality:Polyclonal
Form:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution:WB (1/500 - 1/1000), IH (1/100 - 1/200), IP (1/10 - 1/100)
Gene Symbol:AKT1
Alternative Names:PKB; RAC; RAC-alpha serine/threonine-protein kinase; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; Proto-oncogene c-Akt; RAC-PK-alpha
Entrez Gene (Human): 207;
Entrez Gene (Mouse): 11651;
Entrez Gene (Rat): 24185;
SwissProt (Human): P31749;
SwissProt (Mouse): P31750;
SwissProt (Rat): P47196;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of AKT expression in A549 (A), mouse brain (B), rat lung (C) whole cell lysates. (Predicted band size: 55 kD; Observed band size: 60 kD)
  • Immunohistochemical analysis of AKT staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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