Datasheet
MSDSDescription：Recombinant rabbit monoclonal antibody to Cathepsin SImmunogen：Recombinant full length protein of human Cathepsin SPurification：The antibody was purified by immunogen affinity chromatography.Clonality：MonoclonalForm：Liquid in PBS, pH 7.4, containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)Gene Symbol：CTSSAlternative Names：Cathepsin S
Entrez Gene (Human)：
1520;
SwissProt (Human)：
P25774;
Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Cathepsin S expression in U87MG (A) whole cell lysates. (Predicted band size: 31; 37 kD; Observed band size: 25 kD) -
Immunohistochemical analysis of Cathepsin S staining in human kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. Tyramide-AF488 (green) was used as the chromogen. The section was then counterstained with DAPI (blue). -
Immunofluorescent analysis of Cathepsin S staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).