Description:Recombinant rabbit monoclonal antibody to HGFLImmunogen:KLH-conjugated synthetic peptide encompassing a sequence within human HGFL protein. The exact sequence is proprietary.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:MonoclonalForm:Liquid in PBS, pH 7.4, containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IH (1/50 - 1/200)Gene Symbol:MST1Alternative Names:D3F15S2; DNF15S2; HGFL; Hepatocyte growth factor-like protein; Macrophage stimulatory protein; Macrophage-stimulating protein; MSP
Entrez Gene (Human):
4485;
Entrez Gene (Mouse):
15235;
SwissProt (Human):
P26927;
SwissProt (Mouse):
P26928;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of HGFL expression in H1792 (A), LO2 (B), Panc1 (C), mouse kidney (D) whole cell lysates. (Predicted band size: 80 kD; Observed band size: 85 kD)
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Immunohistochemical analysis of HGFL staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.