Description:Recombinant rabbit monoclonal antibody to Pumilio 1Immunogen:KLH-conjugated synthetic peptide encompassing a sequence within human Pumilio 1 protein. The exact sequence is proprietary.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:MonoclonalForm:Liquid in PBS, pH 7.4, containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IH (1/50 - 1/200)Gene Symbol:PUM1Alternative Names:KIAA0099; PUMH1; Pumilio homolog 1; HsPUM; Pumilio-1
Entrez Gene (Human):
9698;
SwissProt (Human):
Q14671;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Pumilio 1 expression in HepG2 (A), K562 (B) whole cell lysates. (Predicted band size: 126 kD; Observed band size: 140 kD)
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Immunohistochemical analysis of Pumilio 1 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.