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CMA4634
  • Western blot analysis of Pumilio 1 expression in HepG2 (A), K562 (B) whole cell lysates. (Predicted band size: 126 kD; Observed band size: 140 kD)
  • Immunohistochemical analysis of Pumilio 1 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Product Name:Recombinant Anti-Pumilio 1 Rabbit mAb
Cat No:CMA4634
Source:Rabbit
Reactivity:H
Applications:WB, IH
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Size
Price(USD)
200 μl
350
100 μl
220
30 μl
110
50 μl
150
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Description:Recombinant rabbit monoclonal antibody to Pumilio 1
Immunogen:KLH-conjugated synthetic peptide encompassing a sequence within human Pumilio 1 protein. The exact sequence is proprietary.
Purification:The antibody was purified by immunogen affinity chromatography.
Clonality:Monoclonal
Form:Liquid in PBS, pH 7.4, containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.
Dilution:WB (1/500 - 1/1000), IH (1/50 - 1/200)
Gene Symbol:PUM1
Alternative Names:KIAA0099; PUMH1; Pumilio homolog 1; HsPUM; Pumilio-1
Entrez Gene (Human): 9698;
SwissProt (Human): Q14671;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of Pumilio 1 expression in HepG2 (A), K562 (B) whole cell lysates. (Predicted band size: 126 kD; Observed band size: 140 kD)
  • Immunohistochemical analysis of Pumilio 1 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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