Description:Recombinant rabbit monoclonal antibody to IDH1Immunogen:KLH-conjugated synthetic peptide encompassing a sequence within human IDH1 protein. The exact sequence is proprietary.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:MonoclonalForm:Liquid in PBS, pH 7.4, containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IH (1/50 - 1/200)Gene Symbol:IDH1Alternative Names:PICD; Isocitrate dehydrogenase [NADP] cytoplasmic; IDH; Cytosolic NADP-isocitrate dehydrogenase; IDP; NADP(+)-specific ICDH; Oxalosuccinate decarboxylase
Entrez Gene (Human):
3417;
Entrez Gene (Mouse):
15926;
Entrez Gene (Rat):
24479;
SwissProt (Human):
O75874;
SwissProt (Mouse):
O88844;
SwissProt (Rat):
P41562;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of IDH1 expression in K562 (A), Hela (B), mouse liver (C), mouse kidney (D), rat liver (E), rat kidney (F) whole cell lysates. (Predicted band size: 46 kD; Observed band size: 45 kD)
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Immunohistochemical analysis of IDH1 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.