Description：Recombinant rabbit monoclonal antibody to RING1bImmunogen：KLH-conjugated synthetic peptide encompassing a sequence within human RING1b protein. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：MonoclonalForm：Liquid in PBS, pH 7.4, containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)Gene Symbol：RNF2Alternative Names：BAP1; DING; HIPI3; RING1B; E3 ubiquitin-protein ligase RING2; Huntingtin-interacting protein 2-interacting protein 3; HIP2-interacting protein 3; Protein DinG; RING finger protein 1B; RING1b; RING finger protein 2; RING finger protein BAP-1 Entrez Gene (Human)： 6045; Entrez Gene (Mouse)： 19821; Entrez Gene (Rat)： 304850; SwissProt (Human)： Q99496; SwissProt (Mouse)： Q9CQJ4; SwissProt (Rat)： Q4KLY4; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of RING1b expression in K562 (A), Jurkat (B), HepG2 (C), mouse liver (D), rat liver (E) whole cell lysates. (Predicted band size: 37 kD; Observed band size: 38 kD)
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  Immunofluorescent analysis of RING1b staining in A375 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).