Datasheet
MSDSDescription：Recombinant rabbit monoclonal antibody to Matrin 3Immunogen：KLH-conjugated synthetic peptide encompassing a sequence within human Matrin 3 protein. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：MonoclonalForm：Liquid in PBS, pH 7.4, containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)Gene Symbol：MATR3Alternative Names：KIAA0723; Matrin-3
Entrez Gene (Human)：
9782;
Entrez Gene (Mouse)：
17184;
Entrez Gene (Rat)：
29150;
SwissProt (Human)：
P43243;
SwissProt (Mouse)：
Q8K310;
SwissProt (Rat)：
P43244;
Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Matrin 3 expression in K562 (A), MCF7 (B), HepG2 (C), mouse liver (D), mouse kidney (E), rat liver (F), rat kidney (G) whole cell lysates. (Predicted band size: 94 kD; Observed band size: 125 kD) -
Immunofluorescent analysis of Matrin 3 staining in A375 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).