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CMA4574
  • Western blot analysis of PSMB10 expression in Jurkat (A), mouse liver (B), rat liver (C), rat kidney (D) whole cell lysates. (Predicted band size: 28 kD; Observed band size: 27 kD)
  • Immunohistochemical analysis of PSMB10 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Product Name:Recombinant Anti-PSMB10 Rabbit mAb
Cat No:CMA4574
Source:Rabbit
Reactivity:H, M, R
Applications:WB, IH
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Size
Price(USD)
200 μl
350
100 μl
220
30 μl
110
50 μl
150
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Description:Recombinant rabbit monoclonal antibody to PSMB10
Immunogen:KLH-conjugated synthetic peptide encompassing a sequence within human PSMB10 protein. The exact sequence is proprietary.
Purification:The antibody was purified by immunogen affinity chromatography.
Clonality:Monoclonal
Form:Liquid in PBS, pH 7.4, containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.
Dilution:WB (1/500 - 1/1000), IH (1/50 - 1/200)
Gene Symbol:PSMB10
Alternative Names:LMP10; MECL1; Proteasome subunit beta type-10; Low molecular mass protein 10; Macropain subunit MECl-1; Multicatalytic endopeptidase complex subunit MECl-1; Proteasome MECl-1; Proteasome subunit beta-2i
Entrez Gene (Human): 5699;
Entrez Gene (Mouse): 19171;
Entrez Gene (Rat): 291983;
SwissProt (Human): P40306;
SwissProt (Mouse): O35955;
SwissProt (Rat): Q4KM35;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of PSMB10 expression in Jurkat (A), mouse liver (B), rat liver (C), rat kidney (D) whole cell lysates. (Predicted band size: 28 kD; Observed band size: 27 kD)
  • Immunohistochemical analysis of PSMB10 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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