Description:Recombinant rabbit monoclonal antibody to PSMB10Immunogen:KLH-conjugated synthetic peptide encompassing a sequence within human PSMB10 protein. The exact sequence is proprietary.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:MonoclonalForm:Liquid in PBS, pH 7.4, containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IH (1/50 - 1/200)Gene Symbol:PSMB10Alternative Names:LMP10; MECL1; Proteasome subunit beta type-10; Low molecular mass protein 10; Macropain subunit MECl-1; Multicatalytic endopeptidase complex subunit MECl-1; Proteasome MECl-1; Proteasome subunit beta-2i
Entrez Gene (Human):
5699;
Entrez Gene (Mouse):
19171;
Entrez Gene (Rat):
291983;
SwissProt (Human):
P40306;
SwissProt (Mouse):
O35955;
SwissProt (Rat):
Q4KM35;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of PSMB10 expression in Jurkat (A), mouse liver (B), rat liver (C), rat kidney (D) whole cell lysates. (Predicted band size: 28 kD; Observed band size: 27 kD)
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Immunohistochemical analysis of PSMB10 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.