Description：Recombinant rabbit monoclonal antibody to MST4Immunogen：KLH-conjugated synthetic peptide encompassing a sequence within human MST4 protein. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：MonoclonalForm：Liquid in PBS, pH 7.4, containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)Gene Symbol：MST4Alternative Names：MASK; Serine/threonine-protein kinase MST4; Mammalian STE20-like protein kinase 4; MST-4; Mst3 and SOK1-related kinase; STE20-like kinase MST4; Serine/threonine-protein kinase MASK Entrez Gene (Human)： 51765; Entrez Gene (Mouse)： 70415; SwissProt (Human)： Q9P289; SwissProt (Mouse)： Q99JT2; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of MST4 expression in H1792 (A), LO2 (B), Panc1 (C), mouse kidney (D), mouse muscle (E), rat kidney (F), rat muscle (G) whole cell lysates. (Predicted band size: 46 kD; Observed band size: 50 kD)
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  Immunohistochemical analysis of MST4 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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  Immunofluorescent analysis of MST4 staining in A375 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).