Description:Recombinant rabbit monoclonal antibody to UAP1Immunogen:KLH-conjugated synthetic peptide encompassing a sequence within human UAP1 protein. The exact sequence is proprietary.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:MonoclonalForm:Liquid in PBS, pH 7.4, containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IH (1/50 - 1/200)Gene Symbol:UAP1Alternative Names:SPAG2; UDP-N-acetylhexosamine pyrophosphorylase; Antigen X; AGX; Sperm-associated antigen 2
Entrez Gene (Human):
6675;
SwissProt (Human):
Q16222;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of UAP1 expression in Jurkat (A), K562 (B), PC3 (C) whole cell lysates. (Predicted band size: 58 kD; Observed band size: 59 kD)
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Immunohistochemical analysis of UAP1 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.