Description：Recombinant rabbit monoclonal antibody to PFKFB3Immunogen：KLH-conjugated synthetic peptide encompassing a sequence within human PFKFB3 protein. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：MonoclonalForm：Liquid in PBS, pH 7.4, containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)Gene Symbol：PFKFB3Alternative Names：6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3; 6PF-2-K/Fru-2,6-P2ase 3; PFK/FBPase 3; 6PF-2-K/Fru-2,6-P2ase brain/placenta-type isozyme; Renal carcinoma antigen NY-REN-56; iPFK-2 Entrez Gene (Human)： 5209; SwissProt (Human)： Q16875; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of PFKFB3 expression in MCF7 (A), Hela (B), K562 (C) whole cell lysates. (Predicted band size: 59 kD; Observed band size: 60 kD)
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  Immunofluorescent analysis of PFKFB3 staining in A375 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).