Description：Recombinant rabbit monoclonal antibody to TRIM25Immunogen：KLH-conjugated synthetic peptide encompassing a sequence within human TRIM25. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：MonoclonalForm：Liquid in PBS, pH 7.4, containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)Gene Symbol：TRIM25Alternative Names：EFP; RNF147; ZNF147; E3 ubiquitin/ISG15 ligase TRIM25; Estrogen-responsive finger protein; RING finger protein 147; Tripartite motif-containing protein 25; Ubiquitin/ISG15-conjugating enzyme TRIM25; Zinc finger protein 147 Entrez Gene (Human)： 7706; SwissProt (Human)： Q14258; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of TRIM25 expression in PC3 (A), THP1 (B), HepG2 (C) whole cell lysates. (Predicted band size: 70 kD; Observed band size: 71 kD)
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  Immunofluorescent analysis of TRIM25 staining in A375 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).