Datasheet
MSDSDescription：Recombinant rabbit monoclonal antibody to Lamin B2Immunogen：KLH-conjugated synthetic peptide encompassing a sequence within human Lamin B2. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：MonoclonalForm：Liquid in PBS, pH 7.4, containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)Gene Symbol：LMNB2Alternative Names：LMN2; Lamin-B2
Entrez Gene (Human)：
84823;
SwissProt (Human)：
Q03252;
Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of Lamin B2 expression in PC3 (A), THP1 (B), HepG2 (C) whole cell lysates. (Predicted band size: 69 kD; Observed band size: 68 kD) -
Immunofluorescent analysis of Lamin B2 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).