Description：Recombinant rabbit monoclonal antibody to SEC14L2Immunogen：KLH-conjugated synthetic peptide encompassing a sequence within human SEC14L2. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：MonoclonalForm：Liquid in PBS, pH 7.4, containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)Gene Symbol：SEC14L2Alternative Names：C22orf6; KIAA1186; KIAA1658; SEC14-like protein 2; Alpha-tocopherol-associated protein; TAP; hTAP; Squalene transfer protein; Supernatant protein factor; SPF Entrez Gene (Human)： 23541; Entrez Gene (Mouse)： 67815; SwissProt (Human)： O76054; SwissProt (Mouse)： Q99J08; SwissProt (Rat)： Q99MS0; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of SEC14L2 expression in PC3 (A), Hela (B), HepG2 (C), mouse liver (D), mouse muscle (E), rat liver (F), rat muscle (G) whole cell lysates. (Predicted band size: 46 kD; Observed band size: 46 kD)
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  Immunofluorescent analysis of SEC14L2 staining in A375 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).