Description:Recombinant rabbit monoclonal antibody to HSPA12AImmunogen:KLH-conjugated synthetic peptide encompassing a sequence within human HSPA12A. The exact sequence is proprietary.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:MonoclonalForm:Liquid in PBS, pH 7.4, containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IH (1/50 - 1/200)Gene Symbol:HSPA12AAlternative Names:KIAA0417; Heat shock 70 kDa protein 12A
Entrez Gene (Human):
259217;
Entrez Gene (Mouse):
73442;
SwissProt (Human):
O43301;
SwissProt (Mouse):
Q8K0U4;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of HSPA12A expression in mouse kidney (A) whole cell lysates. (Predicted band size: 74 kD; Observed band size: 75 kD)
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Immunohistochemical analysis of HSPA12A staining in mouse brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. Tyramide-AF488 (green) was used as the chromogen. The section was then counterstained with DAPI (blue).